RT-PCR

Reverse transcription-polymerase chain reaction is a molecular technique commonly called RT-PCR. PCR stands for polymerase chain reaction, a laboratory method used to amplify specific regions of DNA for diagnosis and analysis in medical research. Reverse transcriptase PCR, instead of DNA, uses mRNA as the starting template. Real-time PCR is a modified version of PCR in which the enzyme called reverse transcriptase is used. This enzyme facilitates the conversion of an RNA sequence into its complementary DNA sequence. RT-PCR works on this principle of reverse transcription. It is a real-time technique that combines reverse transcription of RNA into DNA and the amplification of target DNA. It’s a qualitative means of detecting a particular RNA sequence with a high degree of accuracy and sensitivity.