HPLC

High performance liquid chromatography is  basically an improvised variety of column chromatography. HPLC is a technique that separates analytes which are dissolved in a liquid. It can separate, identify, purify, and quantify the compounds that are present in any liquid sample. It is used to provide information on the composition of Drug-related samples. HPLC is the most accurate analytical method widely used for the quantitative as well as qualitative analysis of drug products and used for the determination of drug product stability. Chromatography can be described as a mass transfer process involving adsorption using a non-polar stationary phase and a mobile polar phase titrating through the column. Its composition and temperature plays a major role in the separation process by influencing the interactions taking place between sample components and sorbent.  HPLC is optimum for the separation of chemical and biological compounds that are non-volatile.